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Ten species of Rhopalostroma are distinguished, based on the size and the morphological characteristics of ascospores and stromata Whalley et al. The Staden Sequence Analysis Package. The Xylariaceae amongst them were identified by using the microscopic methods described by Stadler et al. The time course of metabolite production was followed by HPLC profiling of samples taken daily for up to 10 days of fermentation, and glucose and pH were determined concurrently. Following centrifugation and resuspension, the ITS region was sequenced through the sequencing service of the faculty of genetics Ludwig-Maximilians-University, Munich , using an ABI capillary sequencer.

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These characters are rarely observed in cultures of Hypoxylon and Annulohypoxylon. The monophyletic origin of Thamnomyces and R. Asci, ascospores and paraphyses; b. Larger amounts of BNT were observed in the extracts from the strmoa, whereas the unknown components that might be azaphilones prevailed in extracts from the perithecial region.

Chemotaxonomic and phylogenetic studies of Thamnomyces Xylariaceae. Thick-walled hyphae observed in melanising cultures; b—e. Stdoma are known with certainty from some species of Hypoxylon and Daldiniabut were to our knowledge never observed in other hypoxyloid Xylariaceae BiscogniauxiaCamilleanor in the xylarioid Xylariaceae i.

A revision of the genus Daldinia. Table 1 Comparison of Xylariaceae with regard to characters relating to stromatal morphology and anatomy and morphology of asci and ascospores that are currently used for delineation of generic boundaries, and characteristic metabolite types produced by representatives of these genera in culture.

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Despite that, both genera appear paraphyletic in their current circumscription for other reasons, as already shown Hsieh et al. Following centrifugation and resuspension, the ITS region was sequenced through the sequencing service of the faculty of genetics Ludwig-Maximilians-University, Munichusing an ABI capillary sequencer. Ruwenzoria, a new genus of the Xylariaceae from Central Africa. In this context, it appeared practical to report unpublished morphological and chemotaxonomic data on Rhopalostroma spp.

The time course of metabolite production was followed by HPLC profiling of samples taken daily for up to 10 days of fermentation, and glucose and pH were determined concurrently. In this context, it has been discussed that Fanw pigments of ancient type material should be confirmed by studies in fresh specimens, wherever possible Stadler et al. Secondary metabolite profiling was carried out using a dual HPLC system, comprising a diode array detector DAD and mass spectrometric MS detectors, the latter providing mass spectra in the positive and negative electrospray ionisation ESI mode, using HPLC-based dereplication library of Xylariaceae metabolites Fawwn et al.

Dtroma holotype specimen in K cf. You must attribute the work in the manner specified by the author or licensor but not in any way that suggests that they endorse you or your use of the work.

The phylogenetic position of Rhopalostroma as inferred from a polythetic approach

Subsequently, this hypothesis was reinforced by studies of their anamorphic fwan It had, however, been possible to obtain cultures from other Xylariaceae e. The isotype specimen of R. Botanical Journal of Scotland Nucleic Acids Research Open in a separate window. Perithecia are embedded in a monostichous layer at the upper part of the stroma, below the convex surface of the semiglobose to subglobose stromatal head, surrounded by characteristic pigment granules.

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Life history studies of Brazilian ascomycetes. Studies in the genus Phylacia Xylariaceae. The genus Rhopalostroma was erected by Hawksworth to accommodate a series of palaeotropical pyrenomycetes that are characterised by producing narrowly obconical, often stipitate stromata.

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ITS primers with enhanced specificity for basidiomycetes applications to the identification of mycorrhizae and rusts. Neither were fanw derivatives 89which are characteristic of Hypoxylon Bitzer et al.

Mycelium composed of fawj hyphae Fig. For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http: Cultures of Rhopalostroma angolense in mL Erlenmeyer flasks supplied with mL of Difco OA after 5 wk a, b and YMG medium after 6 wk crespectively, showing stromatal primordia c ; d, e.

The only extant viable culture of Rhopalostroma was obtained from the perithecial contents of a stromz of R. Find articles by S. Patel IMI as Rhopalostroma sp.